FRET imaging microscopy

Brian Herman; Mao Sun; Atsushi Masuda; Hans C. Gerritsen; Victoria Frohlich Centonze

doi:10.1117/12.470683

17 June 2002 FRET imaging microscopy

Brian Herman, Mao Sun, Atsushi Masuda, Hans C. Gerritsen, Victoria Frohlich Centonze

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470683
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

Fluorescence Resonance Energy Transfer (FRET) Microscopy has been finding substantial utility in the measurement of a number of biological processes. Most microscopic techniques that have been developed to monitor FRET measure changes in the donor and acceptor emission or fluorescent lifetime of the donor. These include measurements of sensitized emission, acceptor photobleaching and fluorescent lifetime imaging (FLI). However, which of these approaches is the best for a given experimental situation and for use with multiphoton microscopy is not clear. Using mutant GFP FRET caspase-2 substrate targeted to mitochondria, we compare FRET efficiencies measured using sensitized emission, acceptor photobleaching and FLI.

Citation Download Citation

Brian Herman, Mao Sun, Atsushi Masuda, Hans C. Gerritsen, and Victoria Frohlich Centonze "FRET imaging microscopy", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470683

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