Highly sensitive chemiluminescence analysis system for medical radiation dose evaluation

Xian Li; Yadong Wei; Da Xing; Yonghong He

doi:10.1117/12.482943

12 September 2002 Highly sensitive chemiluminescence analysis system for medical radiation dose evaluation

Xian Li, Yadong Wei, Da Xing, Yonghong He

Proceedings Volume 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment; (2002) https://doi.org/10.1117/12.482943
Event: Photonics Asia, 2002, Shanghai, China

Abstract

Chemiluminescence (CL) analysis technique is an important method in biological and medical diagnosis. In this research work, we present a novel system to detect and analyze the CL during the actinotheraphy to evaluate the radiation dose according to the principle of the cell stress response to ionizing radiation. The system consists of a sample chamber, a photomultiplier tube (PMT) detector, an amplifier, a discriminator, and a pulses counter. The PMT was cooled to reduce the thermal noise. With the single photon counting technique, high sensitivity, signal-to-noise ratio and stability was achieved. The sample chamber, which was driven by a step motor, has six sample cells for control experiments. A personal computer dominates the data acquisition and the control of the system through the newly implemented universal serial bus interface. There is also a peristaltic pump for auto-injecting the chemical reagent solution. The software developed on the LabVIEW platform makes the system with high automation. With the system, radiation dose could be quantified by the CL intensity from the cell stress response to ionizing radiation. Leukocytes, CCRF cell line and a CL probe MCLA were used to test the reliability ofthis system. The relationship between the 60Co irradiation doses and the intensity ofthe MCLA CL were obtained, which showed that this system could image the CL properly.

Citation Download Citation

Xian Li, Yadong Wei, Da Xing, and Yonghong He "Highly sensitive chemiluminescence analysis system for medical radiation dose evaluation", Proc. SPIE 4916, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment, (12 September 2002); https://doi.org/10.1117/12.482943

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KEYWORDS

Luminescence

Blood

Chemiluminescence

Single photon

Biological research

Radiation effects

Data acquisition

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