Imaging of cell layers by differential phase contrast optical coherence microscopy

Christoph K. Hitzenberger; Markus Sticker; Michael Pircher; Erich Gotzinger; Harald Sattmann; Adolf Friedrich Fercher

doi:10.1117/12.477890

8 July 2003 Imaging of cell layers by differential phase contrast optical coherence microscopy

Christoph K. Hitzenberger, Markus Sticker, Michael Pircher, Erich Gotzinger, Harald Sattmann, Adolf Friedrich Fercher

Proceedings Volume 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII; (2003) https://doi.org/10.1117/12.477890
Event: Biomedical Optics, 2003, San Jose, CA, United States

Abstract

Optical coherence tomography (OCT) measures and images the backscattering potential of transparent and semi-transparent samples. Recently, we reported on an extension of OCT with a differential phase contrast (DPC) technique. We have improved the technique and developed a DPC optical coherence microscope (OCM) which allows to image sub-wavelength optical path differences occurring between a narrow beam probing a sample and its surrounding. This allows the visualization of small transversal refractive index variations close to a selected interface. We report on the method and present first images of a single cell layer. The cells act as phase objects, and by imaging the phase properties the contrast is improved as compared to intensity images.

Citation Download Citation

Christoph K. Hitzenberger, Markus Sticker, Michael Pircher, Erich Gotzinger, Harald Sattmann, and Adolf Friedrich Fercher "Imaging of cell layers by differential phase contrast optical coherence microscopy", Proc. SPIE 4956, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VII, (8 July 2003); https://doi.org/10.1117/12.477890

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