Modeling and validation of a molded polycarbonate continuous-flow polymerase chain reaction device

Michael W. Mitchell; Xuezhu Liu; Yannick Bejat; Dimitris E. Nikitopoulos; Steven A. Soper; Michael C. Murphy

doi:10.1117/12.478142

17 January 2003 Modeling and validation of a molded polycarbonate continuous-flow polymerase chain reaction devic

Michael W. Mitchell, Xuezhu Liu, Yannick Bejat, Dimitris E. Nikitopoulos, Steven A. Soper, Michael C. Murphy

Proceedings Volume 4982, Microfluidics, BioMEMS, and Medical Microsystems; (2003) https://doi.org/10.1117/12.478142
Event: Micromachining and Microfabrication, 2003, San Jose, CA, United States

Abstract

A continuous flow polymerase chain reaction (CFPCR) system was designed, fabricated from molded polycarbonate, and tested. Finite element modeling was used to simulate the thermal and Microfluidic response of the system. The mold insert for the initial prototypes was fabricated using the X-ray LIGA microfabrication process and device components produced by hot embossing polycarbonate. Commercial thin film heaters under PID control were used to supply the necessary heat flux to maintain the steady-state temperatures in the PCR. The simulated transient temperature response at start up was compared to the experimental response. The simulated steady state temperature profile along the channel generated by the finite element analysis was compared to the experimental temperature profile displayed by liquid crystals. Experimental and simulated results were within 5% of each other, validating the thermal design of the CFPCR device.

Citation Download Citation

Michael W. Mitchell, Xuezhu Liu, Yannick Bejat, Dimitris E. Nikitopoulos, Steven A. Soper, and Michael C. Murphy "Modeling and validation of a molded polycarbonate continuous-flow polymerase chain reaction devic", Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); https://doi.org/10.1117/12.478142

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