Optical biosensing transducer based on silicon waveguide structure coated with polyelectrolyte nano layers

Saharudin Haron; Alexey V. Nabok; Asim Kumar Ray

doi:10.1117/12.498825

18 April 2003 Optical biosensing transducer based on silicon waveguide structure coated with polyelectrolyte nano layers

Saharudin Haron, Alexey V. Nabok, Asim Kumar Ray

Author Affiliations +

Proceedings Volume 5119, Bioengineered and Bioinspired Systems; (2003) https://doi.org/10.1117/12.498825
Event: Microtechnologies for the New Millennium 2003, 2003, Maspalomas, Gran Canaria, Canary Islands, Spain

Abstract

An optical biosensor based on attenuation of the light intensity during multiple reflections in a planar waveguide has been developed for water pollution monitoring. The planar waveguide consists of a 190 nanometer thick silicon nitride (Si₃N₄) core layer sandwiched between 1.5 micrometer thick silicon dioxide (SiO₂) cladding layers. Composite polyelectrolyte self-assembled membranes containing Cyclotetrachromotropylene (CTCT) as an indicator and enzymes, such as Urease or Acetylcholine Esterase (AChE) were deposited on top of silicon nitride core layer within a 4 × 6 mm sensing window. Experimental studies on the light propagation through the planar waveguide show the advantages of this method over conventional UV-visible absorption spectroscopy. It was found that the planar waveguide sensitivity is higher by several orders of magnitude than that for UV-visible absorption spectroscopy. The respective enzyme reactions as well as their inhibition by heavy metal ions were studied by monitoring the light intensity in the planar waveguide. Cadmium (Cd²⁺) and lead (Pb²⁺) ions were registered in very low concentrations down to 1 ppb with the planar waveguide transducer. The enzymes used were inhibited differently by the above pollutants, which is promising for the development of enzyme sensor arrays.

Citation Download Citation

Saharudin Haron, Alexey V. Nabok, and Asim Kumar Ray "Optical biosensing transducer based on silicon waveguide structure coated with polyelectrolyte nano layers", Proc. SPIE 5119, Bioengineered and Bioinspired Systems, (18 April 2003); https://doi.org/10.1117/12.498825

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