In vivo quantitative near-infrared spectroscopy in skeletal muscle and bone during rest and isometric exercise

Manfred Klasing; Jochen Zange

doi:10.1117/12.499818

15 October 2003 In vivo quantitative near-infrared spectroscopy in skeletal muscle and bone during rest and isometric exercise

Manfred Klasing, Jochen Zange

Author Affiliations +

Proceedings Volume 5138, Photon Migration and Diffuse-Light Imaging; (2003) https://doi.org/10.1117/12.499818
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

In this study quantitative near-infrared-spectroscopy (NIRS) was investigated as a potential tool to measure local O₂ consumption (mVO₂) in human bone (tibia) in comparison with muscle (musculus tibialis anterior). Both tissues were examined at rest and during 80% maximum voluntary isometric muscle contraction. Fifteen subjects were tested. Local variations in oxy-hemoglobin (O₂Hb), desoxy-hemoglobin (HHb), and total hemoglobin (tHb) were investigated with a continuously operating NIRS system. mVO₂ was determined in phases of applied arterial occlusion. At rest mVO₂ was five times higher in muscle than in bone. However, both mVO₂ values showed a distinct correlation with skin-fold thickness. At rest and only in bone we recorded a periodical variation of O₂Hb. HHb was almost constant. This variation of O₂Hb and the resulting variation in tHb indicated chances in blood volume, which are not compatible with the solid nature of bone. During muscle contraction, mVO₂ in muscle increased about twenty fold. As expected, mVO₂ in bone did not significantly increase during muscle contraction. In conclusion, NIRS was confirmed as a valid method to determine the excess mVO₂ in muscle during contraction. For mVO₂ measurements in bone more sophisticated localization techniques are required to separate the effects derived from bone and skin.

Citation Download Citation

Manfred Klasing and Jochen Zange "In vivo quantitative near-infrared spectroscopy in skeletal muscle and bone during rest and isometric exercise", Proc. SPIE 5138, Photon Migration and Diffuse-Light Imaging, (15 October 2003); https://doi.org/10.1117/12.499818

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