Hyperspectral imaging of cells: toward real-time pathological assessment

Stavros G. Demos; Rajen Ramsamooj M.D.

doi:10.1117/12.508097

12 December 2003 Hyperspectral imaging of cells: toward real-time pathological assessment

Stavros G. Demos, Rajen Ramsamooj M.D.

Proceedings Volume 5201, Photonic Devices and Algorithms for Computing V; (2003) https://doi.org/10.1117/12.508097
Event: Optical Science and Technology, SPIE's 48th Annual Meeting, 2003, San Diego, California, United States

Abstract

The goal of this work is to develop micro-scale noninvasive photonic instrumentation and techniques that will enable real-time imaging and monitoring of microstructures and cells in tissues. We utilize a hyperspectral microscope to explore the differences of native fluorescence and polarized light scattering from cellular components using various excitation wavelengths. The key optical “signature” characteristics that differentiate the various cellular components are used to obtain composite images that highlight their presence and the relative concentration of various tissue chromophores. The sensitivity has been optimized in our specially designed instrumentation so that image acquisition times are very short for real-time application in a clinical setting. This technology is not invasive to the cells and therefore it can be used to monitor their function while they are still alive.

Citation Download Citation

Stavros G. Demos and Rajen Ramsamooj M.D. "Hyperspectral imaging of cells: toward real-time pathological assessment", Proc. SPIE 5201, Photonic Devices and Algorithms for Computing V, (12 December 2003); https://doi.org/10.1117/12.508097

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