Electrochemiluminescence as a tool for microscopy at the nanoscale

Alan Lee; Peter J. Burke; James P. Brody

doi:10.1117/12.530943

1 June 2004 Electrochemiluminescence as a tool for microscopy at the nanoscale

Alan Lee, Peter J. Burke, James P. Brody

Proceedings Volume 5331, Nanobiophotonics and Biomedical Applications; (2004) https://doi.org/10.1117/12.530943
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

The particular form of electrochemiluminescence (ECL) used for analytical assays relies upon the discovery that tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)₃²⁺] emits a 620 nm photon when adjacent to an electrode held at about one volt relative to Ag/AgCl. This reaction occurs within nanometers of the electrode. The enormous economic investment in nanoscale lithography tools is leading to tools capable of routinely producing 32 nm features by 2009. We propose that these two technologies could be combined to produce a nanoscale microscopy system. We constructed a macroscopic test-bed and performed tests on it to explore the feasibility of such a system. We tested an ECL solution containing 1 mM Ru(bpy)₃²⁺ 0.2 mM ammonium oxalate monohydrate in a 0.1 M ammonium acetate buffer at pH 5.0. Using this solution, we found that the ECL light was most intense at an applied voltage of 1.6 Volts, that the effect had excellent reproducibility and that the time to reach maximum intensity was several seconds after applying a voltage.

Citation Download Citation

Alan Lee, Peter J. Burke, and James P. Brody "Electrochemiluminescence as a tool for microscopy at the nanoscale", Proc. SPIE 5331, Nanobiophotonics and Biomedical Applications, (1 June 2004); https://doi.org/10.1117/12.530943

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