Advanced time-correlated single photon counting techniques for spectroscopy and imaging in biomedical systems

Wolfgang Becker; Axel Bergmann; Giovanni Luca Biscotti; Angelika Rueck

doi:10.1117/12.529143

1 June 2004 Advanced time-correlated single photon counting techniques for spectroscopy and imaging in biomedical systems

Wolfgang Becker, Axel Bergmann, Giovanni Luca Biscotti, Angelika Rueck

Author Affiliations +

Proceedings Volume 5340, Commercial and Biomedical Applications of Ultrafast Lasers IV; (2004) https://doi.org/10.1117/12.529143
Event: Lasers and Applications in Science and Engineering, 2004, San Jose, Ca, United States

Abstract

Time-correlated single photon counting (TCSPC) is based on the detection of single photons of a periodic light signal, measurement of the detection time of the photons, and the build-up of the photon distribution versus the time in the signal period. TCSPC achieves a near ideal counting efficiency and transit-time-spread-limited time resolution for a given detector. The drawback of traditional TCSPC is the low count rate, long acquisition time, and the fact that the technique is one-dimensional, i.e. limited to the recording of the pulse shape of light signals. We present an advanced TCSPC technique featuring multi-dimensional photon acquisition and a count rate close to the capability of currently available detectors. The technique is able to acquire photon distributions versus wavelength, spatial coordinates, and the time on the ps scale, and to record fast changes in the fluorescence lifetime and fluorescence intensity of a sample. Biomedical applications of advanced TCSPC techniques are time-domain optical tomography, recording of transient phenomena in biological systems, spectrally resolved fluorescence lifetime imaging, FRET experiments in living cells, and the investigation of dye-protein complexes by fluorescence correlation spectroscopy. We demonstrate the potential of the technique for selected applications.

Citation Download Citation

Wolfgang Becker, Axel Bergmann, Giovanni Luca Biscotti, and Angelika Rueck "Advanced time-correlated single photon counting techniques for spectroscopy and imaging in biomedical systems", Proc. SPIE 5340, Commercial and Biomedical Applications of Ultrafast Lasers IV, (1 June 2004); https://doi.org/10.1117/12.529143

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available