Velocity imaging in microfluidic devices using fluorescence correlation spectroscopy

Kalyan K Kuricheti; Volker Buschmann; Paul Brister; Kenneth D Weston

doi:10.1117/12.532747

23 December 2003 Velocity imaging in microfluidic devices using fluorescence correlation spectroscopy

Kalyan K Kuricheti, Volker Buschmann, Paul Brister, Kenneth D Weston

Proceedings Volume 5345, Microfluidics, BioMEMS, and Medical Microsystems II; (2003) https://doi.org/10.1117/12.532747
Event: Micromachining and Microfabrication, 2004, San Jose, California, United States

Abstract

In this paper we present and demonstrate a technique for mapping fluid flow rates in microfluidic systems with sub-micron resolution using confocal microscopy in conjunction with fluorescence correlation spectroscopy. Velocity profiles and velocity images of the fluid within poly(dimethylsiloxane)-glass microchannels are presented and analyzed. Flow velocities ranging from a few μm/s to a few cm/s can be recorded using nanometer-scale fluorescent polymer spheres as fluid motion tracers. The method is applied to mapping the hydrodynamic flow velocity in complex geometries. This is, to our knowledge, the first report of FCS for producing 2-dimensional velocity maps.

Citation Download Citation

Kalyan K Kuricheti, Volker Buschmann, Paul Brister, and Kenneth D Weston "Velocity imaging in microfluidic devices using fluorescence correlation spectroscopy", Proc. SPIE 5345, Microfluidics, BioMEMS, and Medical Microsystems II, (23 December 2003); https://doi.org/10.1117/12.532747

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