Proposal of spectroscopy-tomography of single cell

Toshiki Yasokawa; Ichirou Ishimaru; Fumikazu Oohira; Ryoji Hyodo; Hiroaki Kobayashi; Akihiro Hayashi; Y. Inoue; Katsumi Ishizaki

doi:10.1117/12.571134

25 October 2004 Proposal of spectroscopy-tomography of single cell

Toshiki Yasokawa, Ichirou Ishimaru, Fumikazu Oohira, Ryoji Hyodo, Hiroaki Kobayashi, Akihiro Hayashi, Y. Inoue, Katsumi Ishizaki

Author Affiliations +

Proceedings Volume 5604, Optomechatronic Micro/Nano Components, Devices, and Systems; (2004) https://doi.org/10.1117/12.571134
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

Currently, diagnosis of cancer is performed by biopsy, whereby medical doctors observe a removed specimen, focusing their attention on morphological changes in the cell sequence and cell nuclei. For early cancer, the only effect is a slight increase in the size of the cell nuclei in comparison with normal cells. Based on medical knowledge, it is presumed that an extremely small amount of a specific protein may be contained in a cell nucleus. We propose spectroscopy-tomography of single cell to measure slight changes in this protein. This technology is composed of two elemental technologies, high spatial resolution spectrometry and a precise single cell rotating method. We propose variable phase-contrast spectrometry as the high spatial resolution spectrometry and proximal two-beam optical tweezers as the precise rotating method. By these methods, we can obtain a 3-dimensional distribution of the cell components to a high spatial resolution. We verified the accuracy of variable phase-contrast spectrometry by measuring the height of a diffraction grating. We confirmed that a microsphere can be rotated by proximal two-beam optical tweezers.

Citation Download Citation

Toshiki Yasokawa, Ichirou Ishimaru, Fumikazu Oohira, Ryoji Hyodo, Hiroaki Kobayashi, Akihiro Hayashi, Y. Inoue, and Katsumi Ishizaki "Proposal of spectroscopy-tomography of single cell", Proc. SPIE 5604, Optomechatronic Micro/Nano Components, Devices, and Systems, (25 October 2004); https://doi.org/10.1117/12.571134

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available