Cell damage during femtosecond optical trapping

Kang-Bin Im; Sumin Han; Hwajoon Park; Dan Jin; Soo-Kie Kim; Beop-Min Kim

doi:10.1117/12.591446

29 March 2005 Cell damage during femtosecond optical trapping

Kang-Bin Im, Sumin Han, Hwajoon Park, Dan Jin, Soo-Kie Kim, Beop-Min Kim

Proceedings Volume 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III; (2005) https://doi.org/10.1117/12.591446
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Microparticles including biological cells were trapped and manipulated using a continuous wave tweezer and a femtosecond laser tweezer. The difference of the optical trapping force between CW and femtosecond optical tweezers increased as the particle size decreased, possibly due to the self-focusing effect of the ultrashort pulses. Also, the white damage spots were generated near the focus during femtosecond optical trapping of biological cells even with extremely low average power. The instantaneous optical damage threshold was measured as a function of the trap depth as well. These results may help to optimize the optical trapping of biological cells using femtosecond lasers.

Citation Download Citation

Kang-Bin Im, Sumin Han, Hwajoon Park, Dan Jin, Soo-Kie Kim, and Beop-Min Kim "Cell damage during femtosecond optical trapping", Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); https://doi.org/10.1117/12.591446

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