Feasibility of detecting peptide phosphorylation by surface enhanced Raman scattering

J. Moger; C.P. Winlove

doi:10.1117/12.644168

27 February 2006 Feasibility of detecting peptide phosphorylation by surface enhanced Raman scattering

J. Moger, C.P. Winlove

Proceedings Volume 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry; 60930G (2006) https://doi.org/10.1117/12.644168
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We report on the feasibility of surface enhanced Raman scattering (SERS) as a highly sensitive technique for detecting peptide phosphorylation. Compared with existing techniques for quantifying peptide phosphorylation, such as high-performance liquid chromatography (HPLC), the short scanning and processing time associated with SERS makes it an attractive alternative for measurement on a near-real-time basis at sub micro-molar concentrations. Using the recently reported drop-coating deposition Raman method we compare our SERS spectra to normal Raman spectra that would otherwise be unobtainable at such low concentration.

Citation Download Citation

J. Moger and C.P. Winlove "Feasibility of detecting peptide phosphorylation by surface enhanced Raman scattering", Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 60930G (27 February 2006); https://doi.org/10.1117/12.644168

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