Quantum dots as resonance energy transfer acceptors for monitoring biological interactions

Niko Hildebrandt; Loïc Charbonnière; Raymond F. Ziessel; Hans-Gerd Löhmannsröben

doi:10.1117/12.660660

14 April 2006 Quantum dots as resonance energy transfer acceptors for monitoring biological interactions

Niko Hildebrandt, Loïc Charbonnière, Raymond F. Ziessel, Hans-Gerd Löhmannsröben

Proceedings Volume 6191, Biophotonics and New Therapy Frontiers; 61910W (2006) https://doi.org/10.1117/12.660660
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

Due to their extraordinary photophysical properties CdSe/ZnS core/shell nanocrystals (quantum dots) are excellent luminescence dyes for fluorescence resonance energy transfer (FRET) systems. By using a supramolecular lanthanide complex with central terbium cation as energy donor, we show that commercially available biocompatible biotinilated quantum dots are excellent energy acceptors in a time-resolved FRET fluoroimmunoassay (FRET-FIA) using streptavidin-biotin binding as biological recognition process. The efficient energy transfer is demonstrated by quantum dot emission sensitization and a thousandfold increase of the nanocrystal luminescence decay time. A Foerster Radius of 90 Å and a picomolar detection limit were achieved in quantum dot borate buffer. Regarding biological applications the influence of bovine serum albumin (BSA) and sodium azide (a frequently used preservative) to the luminescence behaviour of our FRET-system is reported.

Citation Download Citation

Niko Hildebrandt, Loïc Charbonnière, Raymond F. Ziessel, and Hans-Gerd Löhmannsröben "Quantum dots as resonance energy transfer acceptors for monitoring biological interactions", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910W (14 April 2006); https://doi.org/10.1117/12.660660

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