SHG microscopy excited by polarization controlled beam for three-dimensional molecular orientation measurement

K. Yoshiki; M. Hashimoto; T. Araki M.D.

doi:10.1117/12.680506

9 September 2006 SHG microscopy excited by polarization controlled beam for three-dimensional molecular orientation measurement

K. Yoshiki, M. Hashimoto, T. Araki M.D.

Author Affiliations +

Proceedings Volume 6290, Laser Beam Shaping VII; 62900F (2006) https://doi.org/10.1117/12.680506
Event: SPIE Optics + Photonics, 2006, San Diego, California, United States

Abstract

We have developed a second-harmonic-generation (SGH) microscope to observe the three-dimensional molecular orientation with three-dimensional high spatial resolution using a polarization mode converter. The mode converter consists of a parallel-aligned nematic-liquid-crystal spatial-light-modulator (PAL-SLM) and quarter-waveplates, and converts a incident linearly polarized beam to orthogonal linearly polarized beams or radially polarized beam. We combined the mode converter with SHG microscope to obtain the local information of the three-dimensional molecular orientation. We demonstrated the detection of three-dimensional molecular orientation of collagen fiber in human Achilles' tendon. For high precision three-dimensional molecular orientation measurement, we propose a technique to calibrate the dependence of SHG detection efficiencies on molecular orientation using a liposome.

Citation Download Citation

K. Yoshiki, M. Hashimoto, and T. Araki M.D. "SHG microscopy excited by polarization controlled beam for three-dimensional molecular orientation measurement", Proc. SPIE 6290, Laser Beam Shaping VII, 62900F (9 September 2006); https://doi.org/10.1117/12.680506

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