Part II: Morphological analysis of embryonic development following femtosecond laser manipulation

V. Kohli; A. Y. Elezzabi

doi:10.1117/12.760082

20 February 2008 Part II: morphological analysis of embryonic development following femtosecond laser manipulation

V. Kohli, A. Y. Elezzabi

Proceedings Volume 6854, Optical Interactions with Tissue and Cells XIX; 685404 (2008) https://doi.org/10.1117/12.760082
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

The zebrafish (Danio rerio) is an attractive model system that has received wide attention for its usefulness in the study of development and disease. This organism represents a closer analog to humans than the common invetebrates Drosophila melanogaster and Caenorhabditis elegans, making this species an ideal model for human health research. Non-invasive manipulation of the zebrafish has been challenging, owing to the outer proteinaceous membrane and multiple embryonic barriers. A novel tool capable of manipulating early cleavage stage embryonic cells would be important for future advancements in medial research and the aquaculture industry. Herein, we demonstrate the laser surgery of early cleavage stage (2-cell) blastomere cells using a range of average laser powers and beam dwell times. Since the novelty of this manipulation tool depends on its non-invasive application, we examined short- and long-term laser-induced developmental defects following embryonic surgery. Laser-manipulated embryos were reared to 2 and 7 days post-fertilization and compared to control embryos at the same developmental stages. Morphological analysis was performed using light microscopy and scanning electron microscopy. Developmental features that were examined included the antero- and dorsal-lateral whole body views of the larvae, the olfactory pit, dorsal, ventral and pectoral fins, notochord, pectoral fin buds, otic capsule, otic vesicle, neuromast patterning, and kinocilia of the olfactory pit rim and cristae of the lateral wall of the ear. Laser-manipulated embryos developed normally relative to the controls, with developmental patterning and morphology at 2 and 7 days indistinguishable from control larvae.

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V. Kohli and A. Y. Elezzabi "Part II: morphological analysis of embryonic development following femtosecond laser manipulation", Proc. SPIE 6854, Optical Interactions with Tissue and Cells XIX, 685404 (20 February 2008); https://doi.org/10.1117/12.760082

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KEYWORDS

Luminescence

Scanning electron microscopy

Femtosecond phenomena

Laser development

Beam controllers

Pulsed laser operation

Cavitation

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