Reflected light imaging of ON and OFF responses in frog retina

Xin-Cheng Yao; Lei Liu; Yang-Guo Li

doi:10.1117/12.809371

23 February 2009 Reflected light imaging of ON and OFF responses in frog retina

Xin-Cheng Yao, Lei Liu, Yang-Guo Li

Proceedings Volume 7161, Photonic Therapeutics and Diagnostics V; 71613I (2009) https://doi.org/10.1117/12.809371
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Using a near infrared (NIR) light flood-illumination imager equipped with a high-speed CCD camera, we demonstrated reflected light imaging of stimulus-evoked retinal ON and OFF responses in isolated, but intact, frog eye. Both fast and slow transient intrinsic optical signals (IOSs) were observed. Fast optical response occurred immediately after the stimulus onset, and correlated tightly with the ON and OFF edges of the visible light stimulus. High resolution images revealed both positive (increasing) and negative (decreasing) IOSs, and dynamic optical change at individual CCD pixels could often exceed 10% of the background light intensity. Our experiment on isolated eye suggests that further development of fast, high resolution fundus imager will allow robust detection of fast IOSs in vivo, and thus allow noninvasive, three-dimensional evaluation of retinal neural function.

Citation Download Citation

Xin-Cheng Yao, Lei Liu, and Yang-Guo Li "Reflected light imaging of ON and OFF responses in frog retina", Proc. SPIE 7161, Photonic Therapeutics and Diagnostics V, 71613I (23 February 2009); https://doi.org/10.1117/12.809371

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