A comparison between coherent and spontaneous Raman scattering for biological imaging

Brandon R. Bachler; Meng Cui; Sarah R. Nichols; Jennifer P. Ogilvie

doi:10.1117/12.831497

1 July 2009 A comparison between coherent and spontaneous Raman scattering for biological imaging

Brandon R. Bachler, Meng Cui, Sarah R. Nichols, Jennifer P. Ogilvie

Proceedings Volume 7367, Advanced Microscopy Techniques; 73670V (2009) https://doi.org/10.1117/12.831497
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

We compare imaging using coherent and spontaneous Raman scattering under biological imaging conditions. We perform spectral domain imaging of polystyrene beads and find comparable signal levels for both methods at excitation powers and concentrations most relevant for biological samples. The critical power at which the two methods provide equivalent signal levels is found to be ~1.3 mW in 10 M polystyrene beads and ~7 mW in 13 M 2-propanol. The low sample concentrations and low excitation power necessary for most biological imaging applications reduce the relative advantages offered by coherent Raman methods.

Citation Download Citation

Brandon R. Bachler, Meng Cui, Sarah R. Nichols, and Jennifer P. Ogilvie "A comparison between coherent and spontaneous Raman scattering for biological imaging", Proc. SPIE 7367, Advanced Microscopy Techniques, 73670V (1 July 2009); https://doi.org/10.1117/12.831497

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