Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: initial investigation ex vivo

Angelos A. Kalitzeos; Azhar Zam; Florian Stelzle; Eckhart G. Hahn; Martin Raithel; Alexandre Douplik

doi:10.1117/12.831771

9 July 2009 Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: initial investigation ex vivo

Angelos A. Kalitzeos, Azhar Zam, Florian Stelzle, Eckhart G. Hahn, Martin Raithel, Alexandre Douplik

Author Affiliations +

Proceedings Volume 7371, Novel Optical Instrumentation for Biomedical Applications IV; 73710N (2009) https://doi.org/10.1117/12.831771
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

In the current study we try to optimise the cancerous/normal tissue contrast perception using white light and autofluorescence image processing. We deconvolved the RGB channels of both white light and autofluorescence images pixel by pixel and pointed out the best contrast enhancement technique, manipulating each channel seperately. By using false-color mapping according to a particular Look-Up Table, the borders between normal and cancerous area of the tissue are better delineated. Cancer tissue identification was confirmed by the histological examination that followed our investigation.

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Angelos A. Kalitzeos, Azhar Zam, Florian Stelzle, Eckhart G. Hahn, Martin Raithel, and Alexandre Douplik "Enhancement of cancerous/normal tissue contrast via combined white light and fluorescence image processing: initial investigation ex vivo", Proc. SPIE 7371, Novel Optical Instrumentation for Biomedical Applications IV, 73710N (9 July 2009); https://doi.org/10.1117/12.831771

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