Label-free and dynamic detection of biomolecular interactions based on surface plasmon resonance imaging for high-throughput microarray applications

Shengyi Chen; Qiang Li; Tao Deng; Guoliang Huang

doi:10.1117/12.845440

28 October 2009 Label-free and dynamic detection of biomolecular interactions based on surface plasmon resonance imaging for high-throughput microarray applications

Shengyi Chen, Qiang Li, Tao Deng, Guoliang Huang

Author Affiliations +

Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 75190R (2009) https://doi.org/10.1117/12.845440
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

We combined SPRi and protein microarray to build a system for dynamic monitoring of interaction between biomolecules. With this system, we achieved label-free, real-time and automatic detection of specific antigen-antibody interactions. Because protein microarray can be high-throughput, and SPRi is able to achieve real-time and simultaneous monitoring of each probe on the microarray, our system has great potential to realize large-scale and dynamic tracing interactions among biomolecules, thus facilitating drug discovery, molecular diagnostics, signal pathway studies and many other fields.

Citation Download Citation

Shengyi Chen, Qiang Li, Tao Deng, and Guoliang Huang "Label-free and dynamic detection of biomolecular interactions based on surface plasmon resonance imaging for high-throughput microarray applications", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75190R (28 October 2009); https://doi.org/10.1117/12.845440

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available