Ultrasound array photoacoustic microscopy for dynamic in vivo 3D imaging

Liang Song; Konstantin Maslov; K. Kirk Shung; Lihong V. Wang

doi:10.1117/12.840582

23 February 2010 Ultrasound array photoacoustic microscopy for dynamic in vivo 3D imaging

Liang Song, Konstantin Maslov, K. Kirk Shung, Lihong V. Wang

Author Affiliations +

Proceedings Volume 7564, Photons Plus Ultrasound: Imaging and Sensing 2010; 756403 (2010) https://doi.org/10.1117/12.840582
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Using realtime ultrasound array photoacoustic microscopy (UA-PAM), we demonstrated the feasibility of noninvasive in vivo imaging of human pulsatile dynamics, as well as 3-D dynamic imaging of sentinel lymph nodes (SLNs) in a murine model. The system, capable of realtime B-scan imaging at 50 Hz and high-speed 3-D imaging, was validated by imaging the subcutaneous microvasculature in rats and humans. After the validation, a human superficial palmar was imaged, and its pulsatile dynamics monitored, with 20-ms B-scan imaging temporal resolution. In addition, noninvasive photoacoustic sentinel lymph node (SLN) mapping with high spatial resolution has the potential to reduce the false negative rate and eliminate the use of radioactive tracers. Upon intra-dermal injection of Evans blue, the system maps SLNs accurately in mice and rats. Furthermore, the ~6 s 3-D imaging temporal resolution offers the capability to quantitatively and noninvasively monitor the dye dynamics in SLNs in vivo through sequential 3-D imaging. The demonstrated capability suggests that high-speed 3-D photoacoustic imaging should facilitate the understanding of the dynamics of various dyes in SLNs, and potentially help identify SLNs with high accuracy. With the results shown in this study, we believe that UA-PAM can potentially enable many new possibilities for studying functional and physiological dynamics in both preclinical and clinical imaging settings.

Citation Download Citation

Liang Song, Konstantin Maslov, K. Kirk Shung, and Lihong V. Wang "Ultrasound array photoacoustic microscopy for dynamic in vivo 3D imaging", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 756403 (23 February 2010); https://doi.org/10.1117/12.840582

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KEYWORDS

In vivo imaging

Imaging systems

Stereoscopy

Ultrasonography

3D image processing

Photoacoustic microscopy

3D modeling

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