Testing possibilities for establishing nanodiamond-DNA-conjugates

A. Pohl; S. Joch; J. Michael; E. Boschke; P. Quenzel; J. Schreiber; V. Lapina; J. Opitz

doi:10.1117/12.889859

14 June 2011 Testing possibilities for establishing nanodiamond-DNA-conjugates

A. Pohl, S. Joch, J. Michael, E. Boschke, P. Quenzel, J. Schreiber, V. Lapina, J. Opitz

Proceedings Volume 8087, Clinical and Biomedical Spectroscopy and Imaging II; 80871T (2011) https://doi.org/10.1117/12.889859
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

We report on the biofunctionalization of nanodiamond surfaces in a two step procedure: chemical modification, resulting in homogeneous and defined surface coating, with following addition of ssDNA. Carboxylation, thymidine coupling and amination methods for chemical modification of diamond surfaces for further functionalization experiments were applied. To enable the coupling process, biomolecules were also chemically modified with functional groups (-NH2). FTIR spectroscopy, fluorescence microscopy and gel electrophoresis were applied for characterizing modified ND particles and bioconjugates and for controlling the coupling success.

Citation Download Citation

A. Pohl, S. Joch, J. Michael, E. Boschke, P. Quenzel, J. Schreiber, V. Lapina, and J. Opitz "Testing possibilities for establishing nanodiamond-DNA-conjugates", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871T (14 June 2011); https://doi.org/10.1117/12.889859

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