Single optical fiber probe for optogenetics

Ryan Falk; Mohammad Habibi; Ramin Pashaie

doi:10.1117/12.907453

9 February 2012 Single optical fiber probe for optogenetics

Ryan Falk, Mohammad Habibi, Ramin Pashaie

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 82251Z (2012) https://doi.org/10.1117/12.907453
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

With the advent of optogenetics, all optical control and visualization of the activity of specific cell types is possible. We have developed a fiber optic based probe to control/visualize neuronal activity deep in the brain of awake behaving animals. In this design a thin multimode optical fiber serves as the head of the probe to be inserted into the brain. This fiber is used to deliver excitation/stimulation optical pulses and guide a sample of the emission signal back to a detector. The major trade off in the design of such a system is to decrease the size of the fiber and intensity of input light to minimize physical damage and to avoid photobleaching/phototoxicity but to keep the S/N reasonably high. Here the excitation light, and the associated emission signal, are frequency modulated. Then the output of the detector is passed through a time-lens which compresses the distributed energy of the emission signal and maximizes the instantaneous S/N. By measuring the statistics of the noise, the structure of the time lens can be designed to achieve the global optimum of S/N. Theoretically, the temporal resolution of the system is only limited by the time lens diffraction limit. By adding a second detector, we eliminated the effect of input light fluctuations, imperfection of the optical filters, and back-reflection of the excitation light. We have also designed fibers and micro mechanical assemblies for distributed delivery and detection of light.

Citation Download Citation

Ryan Falk, Mohammad Habibi, and Ramin Pashaie "Single optical fiber probe for optogenetics", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82251Z (9 February 2012); https://doi.org/10.1117/12.907453

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