The gene ten-1 contributes to axon regeneration accuracy following femtosecond laser axotomy in C. elegans

Dylan Troy Stevens; Manoj Mathew; Mattias Goksör; Marc Pilon

doi:10.1117/12.928936

10 October 2012 The gene ten-1 contributes to axon regeneration accuracy following femtosecond laser axotomy in C. elegans

Dylan Troy Stevens, Manoj Mathew, Mattias Goksör, Marc Pilon

Author Affiliations +

Proceedings Volume 8458, Optical Trapping and Optical Micromanipulation IX; 84582P (2012) https://doi.org/10.1117/12.928936
Event: SPIE NanoScience + Engineering, 2012, San Diego, California, United States

Abstract

The precise cutting of axons in C. elegans using short laser pulses permits the investigation of parameters that may influence axonal regeneration. This study began by building and optimizing a femtosecond laser axotomy setup that we first used to monitor the effect of cutting axons near or far from the cell body of the PLM mechanosensory neurons in C. elegans. To assess regeneration, we developed a scoring system where the angle between the regenerating trajectory and its direct line to the target is measured; we called this measurement the "angle of regeneration". The results indicate that axons cut near the cell body regenerate better than those cut far from the cell body but nearer their target. The role of teneurins, which are transmembrane proteins with a large extracellular domain that are thought to regulate the remodelling of the extracellular matrix, has not yet been explored as a potential contributor to axon regeneration. We cut PLM axons in wild-type or ten-1 mutant worms, and measured the angle of regeneration 48 hours later, and the frequency of reconnection to the target. Our results show that functional ten-1 contributes to successful axon regeneration.

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Dylan Troy Stevens, Manoj Mathew, Mattias Goksör, and Marc Pilon "The gene ten-1 contributes to axon regeneration accuracy following femtosecond laser axotomy in C. elegans", Proc. SPIE 8458, Optical Trapping and Optical Micromanipulation IX, 84582P (10 October 2012); https://doi.org/10.1117/12.928936

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KEYWORDS

Axons

Neurons

Femtosecond phenomena

Microsurgery

Laser cutting

Confocal microscopy

Microscopes

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