High-speed multispectral confocal imaging

Gary E. Carver; Sarah A. Locknar; William A. Morrison; Daniel L. Farkas

doi:10.1117/12.2001883

22 February 2013 High-speed multispectral confocal imaging

Gary E. Carver, Sarah A. Locknar, William A. Morrison, Daniel L. Farkas

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 858715 (2013) https://doi.org/10.1117/12.2001883
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

A new approach for generating high-speed multispectral images has been developed. The central concept is that spectra can be acquired for each pixel in a confocal spatial scan by using a fast spectrometer based on optical fiber delay lines. This concept merges fast spectroscopy with standard spatial scanning to create datacubes in real time. The spectrometer is based on a serial array of reflecting spectral elements, delay lines between these elements, and a single element detector. The spatial, spectral, and temporal resolution of the instrument is described, and illustrated by multispectral images of laser-induced autofluorescence in biological tissues.

Citation Download Citation

Gary E. Carver, Sarah A. Locknar, William A. Morrison, and Daniel L. Farkas "High-speed multispectral confocal imaging ", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 858715 (22 February 2013); https://doi.org/10.1117/12.2001883

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