Study of virus by Raman spectroscopy

K. Moor; H. Kitamura; K. Hashimoto; M. Sawa; B. B. Andriana; K. Ohtani; T. Yagura; H. Sato

doi:10.1117/12.2004476

22 February 2013 Study of virus by Raman spectroscopy

K. Moor, H. Kitamura, K. Hashimoto, M. Sawa, B. B. Andriana, K. Ohtani , T. Yagura, H. Sato

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85871X (2013) https://doi.org/10.1117/12.2004476
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Problem of viruses is very actual for nowadays. Some viruses, which are responsible for human of all tumors, are about 15 %. Main purposes this study, early detection virus in live cell without labeling and in the real time by Raman spectroscopy. Micro Raman spectroscopy (mRs) is a technique that uses a Raman spectrometer to measure the spectra of microscopic samples. According to the Raman spectroscopy, it becomes possible to study the metabolites of a live cultured cell without labeling. We used mRs to detect the virus via HEK 293 cell line-infected adenovirus. We obtained raman specters of lives cells with viruses in 24 hours and 7 days after the infection. As the result, there is some biochemical changing after the treatment of cell with virus. One of biochemical alteration is at 1081 cm^-1. For the clarification result, we use confocal fluorescent microscopy and transmission electron microscopy (TEM).

Citation Download Citation

K. Moor, H. Kitamura, K. Hashimoto, M. Sawa, B. B. Andriana, K. Ohtani , T. Yagura, and H. Sato "Study of virus by Raman spectroscopy", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871X (22 February 2013); https://doi.org/10.1117/12.2004476

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