Paper
9 March 2013 Integrated liquid jet waveguide for fluorescence spectroscopy on chip
Author Affiliations +
Abstract
An optofluidic jet waveguide for on chip fluorescence analysis is presented. The waveguide consists of an high speed water jet produced by means of a micro-channel coupled with a multimode optical fiber collecting the fluorescence opportunely excited. The liquid jet acts, at the same time, as the solution to analyse and as an optical waveguide. This configuration allows a strong reduction of the scattering and fluorescence of non analyte substances enabling a very low limit of detection (LOD). The integrated device is fabricated by PMMA micro-machining allowing a self-alignment between the liquid jet waveguide and the optical fiber used to deliver the fluorescence to the detector. The performance of the system has been tested on Cy5 water solutions and LOD of 2.56 nM has been obtained. A proof-of-concept of filter-free measurements has been performed demonstrating that fluorescence measurements can be performed also by using a photodiode with an LOD of 6.11 nM.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Gianluca Persichetti, Genni Testa, and Romeo Bernini "Integrated liquid jet waveguide for fluorescence spectroscopy on chip", Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 861502 (9 March 2013); https://doi.org/10.1117/12.2005837
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Waveguides

Liquids

Luminescence

Sensors

Water

Optical fibers

Fluorescence spectroscopy

RELATED CONTENT


Back to Top