Evaluation of the effect of a CO2 laser and fluoride on the reduction of
carious lesions progression in primary teeth: an in vitro study

Bruna R. Zancopé; Marina Moron C. Cesar; Lidiany Karla A. Rodrigues; Marinês Nobre-dos-Santos

doi:10.1117/12.2037284

18 February 2014 Evaluation of the effect of a CO₂ laser and fluoride on the reduction of carious lesions progression in primary teeth: an in vitro study

Bruna R. Zancopé, Marina Moron C. Cesar, Lidiany Karla A. Rodrigues, Marinês Nobre-dos-Santos

Author Affiliations +

Proceedings Volume 8929, Lasers in Dentistry XX; 89290K (2014) https://doi.org/10.1117/12.2037284
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

This study aimed at investigating if CO2 laser irradiation (λ =10.6μm - 11.3 J/cm²) combined with fluoridated products, enhances the CaF₂ formation on enamel surface and inhibits lesion progression of demineralized primary enamel. Thus, 135 demineralized primary enamel specimens (DES) were allocated to 9 groups (n=15) as follows: 1- DES only, 2- DES + pH cycling (control), 3- 1.23% acidulated phosphate fluoride gel (APF), 4- 1.23% fluoride foam (FF), 5- 5% fluoride varnish (FV), 6- CO₂ Laser (L), 7 - Laser during APF application, 8-Laser during FF application and 9-Laser during FV application. Except for the demineralized enamel group, all specimens were submitted to a 7 day pH cycling regime. The knoop hardness number (KHN) was determined by cross-sectional microhardness analysis. After treatments application, three specimens of each group had their surface examined for CaF₂ formation by scanning electron microscopy (SEM). The data was analyzed by ANOVA and Student's t-test (α= 0.05). Enamel mineral loss (ΔS) for groups 1 to 9 were respectively,(8,676.28±1,077.46b),(12,419.54±1,050.21a),(8,156.80±1,279.90b),(8,081.32±1,019.69b),(8,820.86±1,805. 99b),(8,723.45±1,167.14b),(9,003.17±796.90b),(8.229,03±961.25b),(9,023.32±1,1069b). The results showed statistically significant difference between control and all treatments groups (p<0.05). However there was no difference among them (p>0.05). SEM observations showed evidences of melting, fusion and calcium fluoride formation on enamel surface. In conclusion, laser irradiation alone or combined with fluoridated products inhibited lesion progression of demineralized primary enamel surface. However, no synergistic effect was observed when CO₂ laser irradiation and fluoridated products application were combined.

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Bruna R. Zancopé, Marina Moron C. Cesar, Lidiany Karla A. Rodrigues, and Marinês Nobre-dos-Santos "Evaluation of the effect of a CO₂ laser and fluoride on the reduction of carious lesions progression in primary teeth: an in vitro study", Proc. SPIE 8929, Lasers in Dentistry XX, 89290K (18 February 2014); https://doi.org/10.1117/12.2037284

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