In vitro influence of photodynamic antimicrobial chemotherapy on staphylococcus aureus by using phenothiazines derivatives associated with laser/LED Light

Susana C. P. S. de Oliveira; Juliana S. C. Monteiro; Gustavo M. Pires-Santos; Fernando J. P. Sampaio D.D.S.; Rafael Araújo Gomes Júnior; Maria F. M. Gesteira D.D.S.; Aldo Brugnera; Fátima A. A. Zanin; Marcos A. Vannier-Santos; Antônio Luiz B. Pinheiro

doi:10.1117/12.2038576

18 February 2014 In vitro influence of photodynamic antimicrobial chemotherapy on staphylococcus aureus by using phenothiazines derivatives associated with laser/LED light

Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Gustavo M. Pires-Santos, Fernando J. P. Sampaio D.D.S., Rafael Araújo Gomes Júnior, Maria F. M. Gesteira D.D.S., Aldo Brugnera, Fátima A. A. Zanin, Marcos A. Vannier-Santos, Antônio Luiz B. Pinheiro

Author Affiliations +

Proceedings Volume 8932, Mechanisms for Low-Light Therapy IX; 89320H (2014) https://doi.org/10.1117/12.2038576
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

The aim of this study was to evaluate the effect of photodynamic antimicrobial chemotherapy (PACT) using phenothiazinium dyes - PTZ irradiated with red laser (λ660nm) or red-orange LED (λ632±2nm) on Staphylococcus aureus in vitro. triplicate tests were performed in 10 groups: control, Laser (L1₊P_- and L2₊P_-) bacterial suspensions were irradiated only with laser energy 2.4 and 4.8 J/cm² respectively, (Led1₊P_- and Led2₊P_-) irradiated only with LED energy 2.4 and 4.8 J/cm² respectively, (L1₊P₊ and L2₊P₊) irradiated with laser in the presence of 1μg/ml of photosensitizer, (Led1₊P₊ and Led2₊P₊) irradiated with LED in the presence of 1μg/ml of photosensitizer and finally (L_-P₊) only in the presence of PTZ dye. Bactericidal effect of the PACT was assessed by counting colony-forming units. The results showed no significant difference on regards different energy densities on group PACT for both lights. PACT groups (L2₊P₊ and Led2₊P₊) compared to the Control showed significant reduction of CFUs. LED/Laser groups (L2₊P_- and Led2₊P_-) compared to control and PTZ groups showed also significant differences as groups LED/Laser (4.8J/cm²) increased the average of CFUs. Although the results of this study have shown a reduction in average number of colonyforming units by the appropriate Laser or LED-dye treatment combination, it this topic requires further investigation.

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Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Gustavo M. Pires-Santos, Fernando J. P. Sampaio D.D.S., Rafael Araújo Gomes Júnior, Maria F. M. Gesteira D.D.S., Aldo Brugnera, Fátima A. A. Zanin, Marcos A. Vannier-Santos, and Antônio Luiz B. Pinheiro "In vitro influence of photodynamic antimicrobial chemotherapy on staphylococcus aureus by using phenothiazines derivatives associated with laser/LED light", Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 89320H (18 February 2014); https://doi.org/10.1117/12.2038576

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