Imaging of neuronal tissue using a prism adjunct

Philip Broadbridge; Adrian Bradu; Gurprit Lall; Adrian Gh. Podoleanu

doi:10.1117/12.2042007

4 March 2014 Imaging of neuronal tissue using a prism adjunct

Philip Broadbridge, Adrian Bradu, Gurprit Lall, Adrian Gh. Podoleanu

Proceedings Volume 8934, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII; 893438 (2014) https://doi.org/10.1117/12.2042007
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We present the use of a prism as an imaging adjunct with a multimodal system of optical coherence tomography and confocal microscopy operating at 1320 nm and 970 nm respectively. A comparison is performed between en-face OCT images acquired using the system and cross section OCT images obtained through a prism inserted into neuronal tissue of an intact ex-vivo murine brain. The en-face images and cross section images are scans of the same area; however each method has shown different aspects, allowing for greater interpretation of the neuronal tissue.

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Philip Broadbridge, Adrian Bradu, Gurprit Lall, and Adrian Gh. Podoleanu "Imaging of neuronal tissue using a prism adjunct", Proc. SPIE 8934, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII, 893438 (4 March 2014); https://doi.org/10.1117/12.2042007

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KEYWORDS

Prisms

Tissues

Optical coherence tomography

Confocal microscopy

Brain

Beam splitters

Tissue optics

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