Spatial compound imaging for fiber-bundle optic microscopy

Gyeong Woo Cheon; Jaepyeong Cha; Jin U. Kang

doi:10.1117/12.2038690

20 February 2014 Spatial compound imaging for fiber-bundle optic microscopy

Gyeong Woo Cheon, Jaepyeong Cha, Jin U. Kang

Proceedings Volume 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV; 893811 (2014) https://doi.org/10.1117/12.2038690
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Coherent fiber bundles with high core density give both flexibility and high resolution to microscopy. Despite of these advantages, fiber bundles inevitably have uncovered region between adjacent cores. The region results in structural artifact known as pixelation effect. Many kinds of image processing techniques have been introduced to remove this pixelation artifact such as frequency domain filter and Gaussian filter. However, these methods fundamentally have limitation because they use the information of adjacent pixels to make up for these uncovered area; therefore, they cannot avoid blurring effect as a result. To overcome this problem, we introduce spatial compound imaging method to overcome this pixelation artifact. The method uses multiple frames taken with small deviation of position. Some parts of these images include information which is devoid of in other images. The total amount of information increase as more images are added up and we can expect the improvement of resolution in the final images. At the same time, the duplicated parts among these images can be averaged to improve SNR ratio. For these improvements, we essentially need sophisticated registration algorithm. The pixelation artifact is troublesome again in registration process because its structural artifacts are strong features shared with whole images. However, we can solve this problem by using reference image and divide the sample images into two parts: effective and ineffective regions. We used effective regions for registration. We used USAF target to evaluate our method and we could get a result that SNR and resolution are both critically increase.

Citation Download Citation

Gyeong Woo Cheon, Jaepyeong Cha, and Jin U. Kang "Spatial compound imaging for fiber-bundle optic microscopy", Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 893811 (20 February 2014); https://doi.org/10.1117/12.2038690

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