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Most traditional biological assays are based on ensemble measurements on cells. Due to lack of the detection
sensitivity and efficiency in sample preparation, analyzing proteins in single cells have been challenging.
Here we describe an assay-on-a-tip platform, and demonstrate an in situ, label-free technique to detect
proteins inside single cells.
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Q. Quan, F. Liang, W. Hong, D. Schaak, M. Loncar, "Nanoscale fiber tip probe for biomedical sensing," Proc. SPIE 9166, Biosensing and Nanomedicine VII, 91660T (27 August 2014); https://doi.org/10.1117/12.2062485