Effect on influenza hemagglutinin protein binding with neutralizing antibody using terahertz spectroscopy technology

Yiwen Sun; Junlan Zhong; Jian Zuo; Cunlin Zhang

doi:10.1117/12.2071098

11 November 2014 Effect on influenza hemagglutinin protein binding with neutralizing antibody using terahertz spectroscopy technology

Yiwen Sun, Junlan Zhong, Jian Zuo, Cunlin Zhang

Author Affiliations +

Proceedings Volume 9275, Infrared, Millimeter-Wave, and Terahertz Technologies III; 927505 (2014) https://doi.org/10.1117/12.2071098
Event: SPIE/COS Photonics Asia, 2014, Beijing, China

Abstract

Terahertz spectroscopy is sensitive to probe several aspects of biological systems. We have reported the terahertz dielectric spectrum is able to identify the type of the charges in the hydrogen-bonded antibodies’ networks in our previous work. Recently we demonstrate a highly sensitive THz-TDS method to monitor binding interaction of influenza hemagglutinin (HA) against its target antibody F10. The terahertz dielectric properties of HA was strongly affected by the presence of a specific antibody. Protein solution concentration or even molecular binding interaction can also affect the terahertz signal. This enables us to detect the specificity and sensitivity of antibody-antigen binding under THz radiation.

Citation Download Citation

Yiwen Sun, Junlan Zhong, Jian Zuo, and Cunlin Zhang "Effect on influenza hemagglutinin protein binding with neutralizing antibody using terahertz spectroscopy technology", Proc. SPIE 9275, Infrared, Millimeter-Wave, and Terahertz Technologies III, 927505 (11 November 2014); https://doi.org/10.1117/12.2071098

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