Multiphoton microscopy in brain imaging

A. L. Allegra Mascaro; L. Silvestri; I. Costantini; L. Sacconi; B. Maco; G. W. Knott; F. S. Pavone

doi:10.1117/12.2084183

5 March 2015 Multiphoton microscopy in brain imaging

A. L. Allegra Mascaro, L. Silvestri, I. Costantini, L. Sacconi, B. Maco, G. W. Knott, F. S. Pavone

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Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 932903 (2015) https://doi.org/10.1117/12.2084183
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Brain imaging is becoming an important field in the frame of the neurophotonics in correlations with other medical ones in neuroscience studying functional and morphological aspects. In this presentation an overview on multi photon imaging of the brain will be presented, together with innovative aspects related to big area imaging and correlative microscopy approaches. Multiphoton imaging applications will be described together with methods to improve the penetration depth and obtain large area detection, or correlating functional aspects in vivo on single neuron with large area, even on whole brain, morphological aspects. Connecting super resolution features at the nanometer level with micro, meso and macroscopic architectures is in fact one of the challenging aspects to understand brain functioning.

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A. L. Allegra Mascaro, L. Silvestri, I. Costantini, L. Sacconi, B. Maco, G. W. Knott, and F. S. Pavone "Multiphoton microscopy in brain imaging", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932903 (5 March 2015); https://doi.org/10.1117/12.2084183

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