Signal enhancement in optical projection tomography via virtual high dynamic range imaging of single exposure

Yujie Yang; Di Dong; Liangliang Shi; Jun Wang; Xin Yang; Jie Tian

doi:10.1117/12.2081678

17 March 2015 Signal enhancement in optical projection tomography via virtual high dynamic range imaging of single exposure

Yujie Yang, Di Dong, Liangliang Shi, Jun Wang, Xin Yang, Jie Tian

Author Affiliations +

Proceedings Volume 9417, Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging; 941724 (2015) https://doi.org/10.1117/12.2081678
Event: SPIE Medical Imaging, 2015, Orlando, Florida, United States

Abstract

Optical projection tomography (OPT) is a mesoscopic scale optical imaging technique for specimens between 1mm and 10mm. OPT has been proven to be immensely useful in a wide variety of biological applications, such as developmental biology and pathology, but its shortcomings in imaging specimens containing widely differing contrast elements are obvious. The longer exposure for high intensity tissues may lead to over saturation of other areas, whereas a relatively short exposure may cause similarity with surrounding background. In this paper, we propose an approach to make a trade-off between capturing weak signals and revealing more details for OPT imaging. This approach consists of three steps. Firstly, the specimens are merely scanned in 360 degrees above a normal exposure but non-overexposure to acquire the projection data. This reduces the photo bleaching and pre-registration computation compared with multiple different exposures in conventional high dynamic range (HDR) imaging method. Secondly, three virtual channels are produced for each projection image based on the histogram distribution to simulate the low, normal and high exposure images used in the traditional HDR technology in photography. Finally, each virtual channel is normalized to the full gray scale range and three channels are recombined into one image using weighting coefficients optimized by a standard eigen-decomposition method. After applying our approach on the projection data, filtered back projection (FBP) algorithm is carried out for 3-dimentional reconstruction. The neonatal wild-type mouse paw has been scanned to verify this approach. Results demonstrated the effectiveness of the proposed approach.

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Yujie Yang, Di Dong, Liangliang Shi, Jun Wang, Xin Yang, and Jie Tian "Signal enhancement in optical projection tomography via virtual high dynamic range imaging of single exposure", Proc. SPIE 9417, Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging, 941724 (17 March 2015); https://doi.org/10.1117/12.2081678

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KEYWORDS

High dynamic range imaging

Image quality

Tomography

Image enhancement

Optical tomography

Projection systems

Reconstruction algorithms

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