Dynamic speckle-interferometer for intracellular processes analyses at high optical magnification

A. A. Baharev; A. P. Vladimirov; A. S. Malygin; Y. A. Mikhailova; I. A. Novoselova; D. I. Yakin; A. V. Druzhinin

doi:10.1117/12.2184622

22 June 2015 Dynamic speckle-interferometer for intracellular processes analyses at high optical magnification

A. A. Baharev, A. P. Vladimirov, A. S. Malygin, Y. A. Mikhailova, I. A. Novoselova, D. I. Yakin, A. V. Druzhinin

Proceedings Volume 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II; 95291G (2015) https://doi.org/10.1117/12.2184622
Event: SPIE Optical Metrology, 2015, Munich, Germany

Abstract

At present work dynamic of biospeckles is used for studying processes occurring in cells which arranged in the one layer. The basis of many diseases is changes in the structural and functional properties of the molecular cells components as caused by the influence of external factors and internal functional disorders. Purpose of work is approbation of speckle-interferometer designed for the analysis of cellular metabolism in individual cells. As a parameter, characterizing the metabolic activity of cells used the value of the correlation coefficient (η) of optical signals proportional to the radiation intensity I, recorded at two points in time t. At 320x magnification for the cell diameter of 20 microns value η can be determined in the area size of 6 microns.

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A. A. Baharev, A. P. Vladimirov, A. S. Malygin, Y. A. Mikhailova, I. A. Novoselova, D. I. Yakin, and A. V. Druzhinin "Dynamic speckle-interferometer for intracellular processes analyses at high optical magnification", Proc. SPIE 9529, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II, 95291G (22 June 2015); https://doi.org/10.1117/12.2184622

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