An automated tool for 3D tracking of single molecules in living cells

L. Gardini; M. Capitanio; F. S. Pavone

doi:10.1117/12.2184340

14 July 2015 An automated tool for 3D tracking of single molecules in living cells

L. Gardini, M. Capitanio, F. S. Pavone

Proceedings Volume 9536, Advanced Microscopy Techniques IV; and Neurophotonics II; 95361B (2015) https://doi.org/10.1117/12.2184340
Event: European Conferences on Biomedical Optics, 2015, Munich, Germany

Abstract

Recently, tremendous improvements have been achieved in the precision of localization of single fluorescent molecules, allowing localization and tracking of biomolecules at the nm level. Since the behaviour of proteins and biological molecules is tightly influenced by the cell’s environment, a growing number of microscopy techniques are moving from in vitro to live cell experiments. Looking at both diffusion and active transportation processes inside a cell requires three-dimensional localization over a few microns range, high SNR images and high temporal resolution (ms order of magnitude). To satisfy these requirements we developed an automated routine that allow 3D tracking of single fluorescent molecules in living cells with nanometer accuracy, by exploiting the properties of the point-spread-function of out-of-focus Quantum Dots bound to the protein of interest.

Citation Download Citation

L. Gardini, M. Capitanio, and F. S. Pavone "An automated tool for 3D tracking of single molecules in living cells", Proc. SPIE 9536, Advanced Microscopy Techniques IV; and Neurophotonics II, 95361B (14 July 2015); https://doi.org/10.1117/12.2184340

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