Biochemical responses of isolated lung CSCs after application of low intensity laser irradiation

Heidi Abrahamse; Anine Crous

doi:10.1117/12.2228902

8 March 2016 Biochemical responses of isolated lung CSCs after application of low intensity laser irradiation

Heidi Abrahamse, Anine Crous

Proceedings Volume 9695, Mechanisms of Photobiomodulation Therapy XI; 96950J (2016) https://doi.org/10.1117/12.2228902
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Studies have shown that using high fluences of Low Intensity Laser Irradiation (HF-LILI) produce apoptotic effects on normal and neoplastic cells. This study aimed to determine whether HF-LILI induce cell death in lung CSCs. Lung CSCs were isolated using the stem cell marker CD 133, characterized using flow cytometry, and applied in experiments which included treatment with LILI at wavelengths of 636, 825 and 1060 nm with fluences ranging from 5 J/cm² to 40 J/cm². Viability and proliferation studies, using Alamar blue assay and adenosine triphosphate luminescence (ATP), indicated an increase when treating lung CSCs with low fluences of 5 - 20 J/cm² and a decrease in viability and proliferation as well as an increase in apoptosis when applying a fluence of 40 J/cm² indicated by flow cytometry using Annexin V and propidium iodide (PI) dyes. Results indicate that LILI, when treating lung CSCs, can induce either a bio-stimulatory or bio-inhibitory effect depending on the wavelength and fluence used. This study indicated successful apoptotic induction of lung CSCs. Future experiments should be able to conclude the exact mechanism behind HF-LILI, which can be used in the targeted treatments of CSC elimination, implementing HF-LILI in the same manner as PDT in the absence of a photosensitizer.

Citation Download Citation

Heidi Abrahamse and Anine Crous "Biochemical responses of isolated lung CSCs after application of low intensity laser irradiation", Proc. SPIE 9695, Mechanisms of Photobiomodulation Therapy XI, 96950J (8 March 2016); https://doi.org/10.1117/12.2228902

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available