Alkyne-tag Raman imaging of bio-active small molecules in live cells

Jun Ando; Almar F. Palonpon; Hiroyuki Yamakoshi; Kosuke Dodo; Satoshi Kawata; Mikiko Sodeoka; Katsumasa Fujita

doi:10.1117/12.2207554

9 December 2015 Alkyne-tag Raman imaging of bio-active small molecules in live cells

Jun Ando, Almar F. Palonpon, Hiroyuki Yamakoshi, Kosuke Dodo, Satoshi Kawata, Mikiko Sodeoka, Katsumasa Fujita

Proceedings Volume 9792, Biophotonics Japan 2015; 979207 (2015) https://doi.org/10.1117/12.2207554
Event: SPIE/OSJ Biophotonics Japan, 2015, Tokyo, Japan

Abstract

Raman microscopy is useful for molecular imaging and analysis of biological specimens. Here, we used alkyne containing a carbon-carbon triple bond as a Raman tag for observing small molecules in live cells. Alkyne tags can maintain original properties of target molecules with providing high chemical specificity owing to its distinct peak in a Raman-silent window of biomolecules. For demonstrations, alkyne-tagged thymidine and coenzyme Q analogue in live cells were visualized with high-spatial resolution. We extended the application of alkyne-tag imaging to visualize cell organelles and specific lipid components in artificial monolayer membranes.

Citation Download Citation

Jun Ando, Almar F. Palonpon, Hiroyuki Yamakoshi, Kosuke Dodo, Satoshi Kawata, Mikiko Sodeoka, and Katsumasa Fujita "Alkyne-tag Raman imaging of bio-active small molecules in live cells", Proc. SPIE 9792, Biophotonics Japan 2015, 979207 (9 December 2015); https://doi.org/10.1117/12.2207554

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