Laser photodamage threshold assessment for human skin imaging by multiphoton microscopy

Kristina Shrestha; Chung-Ho Sun; Amanda Durkin; Mihaela Balu

doi:10.1117/12.2610054

7 March 2022 Laser photodamage threshold assessment for human skin imaging by multiphoton microscopy

Kristina Shrestha, Chung-Ho Sun, Amanda Durkin, Mihaela Balu

Author Affiliations +

Proceedings Volume PC11934, Photonics in Dermatology and Plastic Surgery 2022; PC119340J (2022) https://doi.org/10.1117/12.2610054
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Our group has recently reported on the development of a fast large area multiphoton exoscope (FLAME) for rapid macroscopic imaging with microscopic resolution of human skin. This imaging platform is based on multiphoton microscopy (MPM) that provide label-free, depth-resolved images by using femtosecond laser pulses. Our goal is to use FLAME for in vivo skin imaging to address unmet needs in clinical research and practice of dermatology. This work evaluates the photodamage threshold induced by the excitation laser for the full Fitzpatrick scale of human skin. The optimum laser power and scanning regimes for safe imaging determined by this study will be used for further in vivo clinical studies.

Conference Presentation

Citation Download Citation

Kristina Shrestha, Chung-Ho Sun, Amanda Durkin, and Mihaela Balu "Laser photodamage threshold assessment for human skin imaging by multiphoton microscopy", Proc. SPIE PC11934, Photonics in Dermatology and Plastic Surgery 2022, PC119340J (7 March 2022); https://doi.org/10.1117/12.2610054

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