Axial spectral encoding for low-cost confocal endomicroscopy

Nachiket Kulkarni; Andrew Masciola; Abhinav Nishant; Kyungjo Kim; Arthur F. Gmitro; Esther E. Freeman; Aggrey Semeere; Miriam Nakalembe; DongKyun Kang

doi:10.1117/12.2609006

7 March 2022 Axial spectral encoding for low-cost confocal endomicroscopy

Nachiket Kulkarni, Andrew Masciola, Abhinav Nishant, Kyungjo Kim, Arthur F. Gmitro, Esther E. Freeman, Aggrey Semeere, Miriam Nakalembe, DongKyun Kang

Author Affiliations +

Proceedings Volume PC11937, Endoscopic Microscopy XVII; PC119370P (2022) https://doi.org/10.1117/12.2609006
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We have developed an axially spectrally-encoded confocal endomicroscope that can visualize multiple depths of the tissue in a single image frame. We developed a custom hyperchromatic objective lens (focal length = 4.8 mm; NA = 0.68) to focus different wavelengths from a broadband light source into different axial depths of the tissue. The confocal endomicroscope achieved lateral and axial resolution of 2 μm and 4 μm, respectively over a lateral field of 468 μm and an axial depth range of 100 μm. The material cost was < $1500. Preliminary confocal images of human tissues in vivo clearly visualized cellular details.

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Nachiket Kulkarni, Andrew Masciola, Abhinav Nishant, Kyungjo Kim, Arthur F. Gmitro, Esther E. Freeman, Aggrey Semeere, Miriam Nakalembe, and DongKyun Kang "Axial spectral encoding for low-cost confocal endomicroscopy", Proc. SPIE PC11937, Endoscopic Microscopy XVII, PC119370P (7 March 2022); https://doi.org/10.1117/12.2609006

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