Visualization and quantification of optogenetic cardiac pacing in Drosophila melanogaster with optical coherence tomography

Andrew L. Lopez III; Elena Gracheva; Hongwu Liang; Chao Zhou

doi:10.1117/12.2606808

7 March 2022 Visualization and quantification of optogenetic cardiac pacing in Drosophila melanogaster with optical coherence tomography

Andrew L. Lopez III, Elena Gracheva, Hongwu Liang, Chao Zhou

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Proceedings Volume PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI; PC119480T (2022) https://doi.org/10.1117/12.2606808
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Optogenetics is a powerful tool that allows tissue specific control with light activation. Here, we use Drosophila melanogaster as a tool to optimize and improve optogenetic cardiac pacing. We have obtained several D. melanogaster strains to test the performance of different opsins,light sensitive proteins, to determine which provide high-fidelity control of the heart with minimal power to activate. Using a lab-built optical coherence tomography (OCT) system integrated with an optogenetic set-up, flies were efficiently tested for performance and fidelity. Using a custom convolutional neural network, 2D+Time pacing images were segmented to quantify functional parameters such heart beat rate, change in lumen area, and heart wall velocity.

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Andrew L. Lopez III, Elena Gracheva, Hongwu Liang, and Chao Zhou "Visualization and quantification of optogenetic cardiac pacing in Drosophila melanogaster with optical coherence tomography", Proc. SPIE PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI, PC119480T (7 March 2022); https://doi.org/10.1117/12.2606808

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KEYWORDS

Optogenetics

Optical coherence tomography

Heart

Visualization

Image segmentation

Control systems

Tissue optics

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