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2 March 2022 DASH – A fast converging algorithm for scattering compensation in nonlinear microscopy
Molly A. May, Nicolas Barré, Kai K. Kummer, Michaela Kress, Monika Ritsch-Marte, Alexander Jesacher
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Proceedings Volume PC11969, Adaptive Optics and Wavefront Control for Biological Systems VIII; PC1196907 (2022) https://doi.org/10.1117/12.2616347
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
We present Dynamic Adaptive Scattering Compensation Holography (DASH) [1], a novel algorithmic approach for indirect wavefront sensing in nonlinear scanning microscopy that is suited for imaging into highly scattering media. DASH utilizes a single phase-only SLM for sculpting a continuously updated aberration corrected beam and a test beam with a particular wavefront shape. The test beam is phase-stepped to evaluate its importance and optimal phase and then added to the corrected. We demonstrate DASH for 2-photon fluorescence imaging of GFP expressing microglia cells in fixed and living mouse hippocampal brain tissue at over 500 µm depth. 1. May, Molly A., Nicolas Barré, Kai K. Kummer, Michaela Kress, Monika Ritsch-Marte, and Alexander Jesacher. "Fast holographic scattering compensation for deep tissue biological imaging." Nature Communications 12, no. 1 (2021): 1-8.
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Molly A. May, Nicolas Barré, Kai K. Kummer, Michaela Kress, Monika Ritsch-Marte, and Alexander Jesacher "DASH – A fast converging algorithm for scattering compensation in nonlinear microscopy", Proc. SPIE PC11969, Adaptive Optics and Wavefront Control for Biological Systems VIII, PC1196907 (2 March 2022); https://doi.org/10.1117/12.2616347
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KEYWORDS
Scattering compensation
Holography
Microscopy
Spatial light modulators
Beam shaping
Tissues
Wavefront sensors
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