Denoising with polychromatic digital holographic microscopy: applications in materials and life sciences

Erik Bélanger; Gabrielle Jess; Mohamed Haouat; Céline Larivière-Loiselle; Émile Rioux-Péllerin; Marie-Ève Crochetière; Jean-Xavier Giroux; Pierre Marquet

doi:10.1117/12.2610503

2 March 2022 Denoising with polychromatic digital holographic microscopy: applications in materials and life sciences

Erik Bélanger, Gabrielle Jess, Mohamed Haouat, Céline Larivière-Loiselle, Émile Rioux-Péllerin, Marie-Ève Crochetière, Jean-Xavier Giroux, Pierre Marquet

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Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC1197211 (2022) https://doi.org/10.1117/12.2610503
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Digital holographic microscopy (DHM) has been used in numerous successful studies in materials and life sciences. It is well known that the light source coherence, useful to generate high-quality interference patterns that encode the phase in an extremely accurate manner, generates coherent noise (CN), which precludes to adequately address some important applications, especially when single-shot fast recording is required. We propose an original approach, called polychromatic DHM, which thanks to the reconstruction of several holograms acquired at different wavelengths provides quasi-CN-free optical path difference images. Preliminary results concerning materials and life sciences will be presented.

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Erik Bélanger, Gabrielle Jess, Mohamed Haouat, Céline Larivière-Loiselle, Émile Rioux-Péllerin, Marie-Ève Crochetière, Jean-Xavier Giroux, and Pierre Marquet "Denoising with polychromatic digital holographic microscopy: applications in materials and life sciences", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC1197211 (2 March 2022); https://doi.org/10.1117/12.2610503

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KEYWORDS

Digital holography

Life sciences

Denoising

Holography

Microscopy

Holography applications

Image processing

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