Moxifloxacin-based extended depth-of-field (EDOF) wide-field microscopy with surface tracking for non-invasive examination of conjunctival goblet cells in awake human subjects

Ki Hean Kim; Jungbin Lee; Seonghan Kim; Suil Jeon; Jieun Yun; Noseung Park; Kyungbin Bae; Chang Ho Yoon

doi:10.1117/12.2652780

17 March 2023 Moxifloxacin-based extended depth-of-field (EDOF) wide-field microscopy with surface tracking for non-invasive examination of conjunctival goblet cells in awake human subjects

Ki Hean Kim, Jungbin Lee, Seonghan Kim, Suil Jeon, Jieun Yun, Noseung Park, Kyungbin Bae, Chang Ho Yoon

Author Affiliations +

Proceedings Volume PC12360, Ophthalmic Technologies XXXIII; PC123600Q (2023) https://doi.org/10.1117/12.2652780
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Mucin secretive conjunctival goblet cells (CGCs) in the eye play important roles in ocular surface homeostasis by forming the mucous layer of the tear film. CGC information is also an important biomarker for diagnosis because CGC loss or dysfunction is observed in various ocular surface diseases. In this study, we developed moxifloxacin-based extended depth-of-field (EDOF) microscopy with surface tracking for non-invasive CGC imaging in awake human subjects. The system had a DOF of 0.8 mm, a field of view (FOV) of 1.3mm x 1.3mm, and imaging speed of 15 fps. The phase detection method was used for real-time surface tracking. Moxifloxacin ophthalmic solution was topically instilled for CGC labeling. Repeated large area imaging of the same conjunctiva in a human subject was demonstrated. MBFM might have the potential for non-invasive CGC examination in patients.

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Ki Hean Kim, Jungbin Lee, Seonghan Kim, Suil Jeon, Jieun Yun, Noseung Park, Kyungbin Bae, and Chang Ho Yoon "Moxifloxacin-based extended depth-of-field (EDOF) wide-field microscopy with surface tracking for non-invasive examination of conjunctival goblet cells in awake human subjects", Proc. SPIE PC12360, Ophthalmic Technologies XXXIII, PC123600Q (17 March 2023); https://doi.org/10.1117/12.2652780

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KEYWORDS

Imaging systems

Microscopy

Human subjects

Animal model studies

Eye

In vivo imaging

Eye models

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