Direct imaging of lipid metabolism in cells and brain organoids by optical photothermal infrared microscopy

Yeran Bai; Stella Glasauer; Carolina M. Camargo; Xinran Tian; Andrew Longhini; Kenneth Kosik; Craig Prater

doi:10.1117/12.2651046

17 March 2023 Direct imaging of lipid metabolism in cells and brain organoids by optical photothermal infrared microscopy

Yeran Bai, Stella Glasauer, Carolina M. Camargo, Xinran Tian, Andrew Longhini, Kenneth Kosik, Craig Prater

Author Affiliations +

Proceedings Volume PC12365, Neural Imaging and Sensing 2023; PC123650A (2023) https://doi.org/10.1117/12.2651046
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Direct imaging of metabolism at sub-cellular resolution provides insights to understand single-cell physiology at spatially resolved manner. Here, we presented a metabolic profiling platform based on Optical Photothermal Infrared (OPTIR) spectroscopic imaging that enables in situ lipid metabolism characterization at sub-cellular resolution. We applied the developed metabolic imaging platform to study lipid synthesis from azide-labeled fatty acids across various systems including neuroglioma cells, human-induced pluripotent stem cells (iPSCs), iPSC-derived microglia cells, and iPSC-derived brain organoids. The specificity of the developed platform was further improved by coupling a fluorescence module that enables cell type specific-metabolic profiling. Collectivity, the proposed metabolic imaging platform opens a vast potential for in situ metabolism characterization at the single-cell level.

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Citation Download Citation

Yeran Bai, Stella Glasauer, Carolina M. Camargo, Xinran Tian, Andrew Longhini, Kenneth Kosik, and Craig Prater "Direct imaging of lipid metabolism in cells and brain organoids by optical photothermal infrared microscopy", Proc. SPIE PC12365, Neural Imaging and Sensing 2023, PC123650A (17 March 2023); https://doi.org/10.1117/12.2651046

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