3D Cell Model monitoring using multimodal optical microscopy imaging system

Hyunji Lee; Jae-Won Choi; Min-Beom Heo; Sang-Won Lee

doi:10.1117/12.2667037

6 March 2023 3D Cell Model monitoring using multimodal optical microscopy imaging system

Hyunji Lee, Jae-Won Choi, Min-Beom Heo, Sang-Won Lee

Proceedings Volume PC12370, Design and Quality for Biomedical Technologies XVI; PC123700A (2023) https://doi.org/10.1117/12.2667037
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

3D cell models that are induced to interact and grow in a 3D environment are formed up to several hundred micrometers. Since cells grow in all directions, an imaging system that can show molecular images using fluorescence as well as morphological structures without damaging the structure of the cell model is needed. We have developed a multimodal optical microscopy imaging system that combines extended depth of focus-optical coherence microscopy (EDOF-OCM) and two-photon fluorescence microscopy (TPFM) for more accurate measurement of 3D cells. This imaging system was able to acquire merged images without moving the spheroids, and the efficiency of the imaging systems was compared using optical coherence tomography (OCT), OCM, and confocal fluorescence microscopy(CFM).

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Hyunji Lee, Jae-Won Choi, Min-Beom Heo, and Sang-Won Lee "3D Cell Model monitoring using multimodal optical microscopy imaging system ", Proc. SPIE PC12370, Design and Quality for Biomedical Technologies XVI, PC123700A (6 March 2023); https://doi.org/10.1117/12.2667037

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KEYWORDS

3D modeling

Imaging systems

3D image processing

Optical microscopy

3D metrology

Fluorescence

Optical coherence tomography

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