Wide-field optical redox imaging with leading-edge detection for assessment of patient-derived cancer organoids (Conference Presentation)

Amani A. Gillette; Shirsa Udgata; Lexie Schmitz; Dustin Deming; Melissa C. Skala

doi:10.1117/12.2650100

16 March 2023 Wide-field optical redox imaging with leading-edge detection for assessment of patient-derived cancer organoids (Conference Presentation)

Amani A. Gillette, Shirsa Udgata, Lexie Schmitz, Dustin Deming, Melissa C. Skala

Author Affiliations +

Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC1239107 (2023) https://doi.org/10.1117/12.2650100
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Patient-derived cancer organoids (PDCOs) are valuable for patient management and drug development. Label-free widefield optical redox ratio (WF-ORR) imaging measures organoid-level treatment response using the autofluorescence intensity of the metabolic co-enzymes NAD(P)H and FAD. We have developed WF-ORR imaging and analysis tools to maximize the accessibility, speed, sensitivity, and reproducibility of drug response measurements in PDCOs. These tools include leading-edge segmentation methods that isolate ORR changes within the proliferating zone of PDCOs, and single organoid tracking that reduces variability within drug screens. Overall, this approach enables rapid, sensitive, and reproducible measurements of treatment response and heterogeneity in PDCOs.

Conference Presentation

Citation Download Citation

Amani A. Gillette, Shirsa Udgata, Lexie Schmitz, Dustin Deming, and Melissa C. Skala "Wide-field optical redox imaging with leading-edge detection for assessment of patient-derived cancer organoids (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC1239107 (16 March 2023); https://doi.org/10.1117/12.2650100

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KEYWORDS

Cancer

Optical imaging

Clinical trials

Drug development

3D image processing

3D metrology

Image segmentation

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