Development of circular scanning optical coherence tomography for volumetric imaging of the long mouse spinal cord (Conference Presentation)

SangJin Lee; Eunji Lee; Kibeom Park; Woonggyu Jung

doi:10.1117/12.2650967

16 March 2023 Development of circular scanning optical coherence tomography for volumetric imaging of the long mouse spinal cord (Conference Presentation)

SangJin Lee, Eunji Lee, Kibeom Park, Woonggyu Jung

Author Affiliations +

Proceedings Volume PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; PC1239113 (2023) https://doi.org/10.1117/12.2650967
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

To collect the volumetric image of the spinal cord using optical coherence tomography, the traditional way is to successively repeat the process of cutting the sample and imaging the revealed part. This common method is quite lengthy and laborious. Here, we designed circular scanning optical coherence tomography that has a customized ring-shaped silver-coated mirror reflecting incoming light in a circular pattern around the spinal cord. The sample is carried through that mirror by the linear stage during image acquisition. The results suggest that our system is suitable for the study of the spinal cord as well as applicable to other bigger samples when adapting clearing methods.

Conference Presentation

Citation Download Citation

SangJin Lee, Eunji Lee, Kibeom Park, and Woonggyu Jung "Development of circular scanning optical coherence tomography for volumetric imaging of the long mouse spinal cord (Conference Presentation)", Proc. SPIE PC12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, PC1239113 (16 March 2023); https://doi.org/10.1117/12.2650967

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KEYWORDS

Spinal cord

Optical coherence tomography

Mirrors

3D image processing

Glasses

Image acquisition

Image processing

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