Unleashing the freedom of optical manipulation for intercellular activities

Bin Dong; Shivam Mahapatra; Robert M. Everly; Chi Zhang; Matthew G. Clark; Karsten Mohn; Seohee Ma

doi:10.1117/12.3002085

13 March 2024 Unleashing the freedom of optical manipulation for intercellular activities

Bin Dong, Shivam Mahapatra, Robert M. Everly, Chi Zhang, Matthew G. Clark, Karsten Mohn, Seohee Ma

Proceedings Volume PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII; PC128460K (2024) https://doi.org/10.1117/12.3002085
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Optical control technologies have been demonstrated with high spatial precision. However, present methods are facing challenges in real-time selection and manipulation. To address these limitations, we present a real-time precision optical control technology (RPOC) which is a close-loop optoelectronic system integrated with a laser scanning confocal fluorescence microscope. This technology allows us to control chemical processes at the target sites in real time with high spatiotemporal precision. Using it, we demonstrated the precise generation of reactive oxygen species (ROS) solely at selected organelles and monitored ROS-induced changes in microtubule polymerization dynamics. We also selectively inhibit tubulin polymerization using RPOC with a photoswitchable inhibitor. Meanwhile, a software-based optical control system is developed for more flexible selections of chemical targets and optical manipulation.

Conference Presentation

Citation Download Citation

Bin Dong, Shivam Mahapatra, Robert M. Everly, Chi Zhang, Matthew G. Clark, Karsten Mohn, and Seohee Ma "Unleashing the freedom of optical manipulation for intercellular activities", Proc. SPIE PC12846, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXII, PC128460K (13 March 2024); https://doi.org/10.1117/12.3002085

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